Gene expression profiling of nodavirus infected-orange spotted grouper Epinephelus coioides
Date Issued
2008
Date
2008
Author(s)
Li, Yu-Chi
Abstract
Following infection, viruses use various mechanisms to control host genes expression. Some of the host genes may be activated or suppressed. And it makes the host cell to be an appropriate place for virus propagation. Meanwhile, the host cells also express genes to against virus infection. Recently, viral nervous necrosis disease, nervous necrosis virus (NNV) causes a great damage to grouper aquaculture in Asia. In order to understand the characteristics of virus infection, we established a λ phage cDNA library of virus infected-host tissue, grouper brain tissue, to screen the differentially-express genes in response to NNV infection, and used 6000 candidates, to construct the microarray chip. The chips was then hybridized with probes prepared from NNV, LPS or poly (I:C) injected-grouper larvae, respectively. fter analyzing two fold changes in at least one of five days of microarray data, the gene numbers of NNV-infected grouper was more than that of LPS- and poly(I:C)-injected. It suggests that more genes were regulated when virus invasion. After comparing these genes sequences with NCBI genetic database, it was observed that these gene’s function can be categorized to transcription, structure, protein degradation, immune, metabolism, transport, protein-protein adhesion, biosynthesis, stress, calcium binding proteins, catalysis, protease, translation, energy or cell cycle. This information could be helpful to further understand the molecular mechanisms in NNV-host interaction and the development of control measures against NNV infection.
Subjects
nodavirus
orange spotted grouper
microarray
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