利用表現基因庫以選殖克雷伯氏桿菌抗 chlorhexidine 相關基因
Other Title
Molecular Cloning of Chlorhexidine Resistance Genes in
Klebsiellapneumoniae Using Expression DNA Library
Klebsiellapneumoniae Using Expression DNA Library
Date Issued
2002
Date
2002
Author(s)
方啟泰
DOI
902320B002151
Abstract
Chlorhexidine is a cationic biguanide
antiseptic agent extensively used for
handwashing in hospital infection control
practice. We constructed a l-Zap II expression library of a chlorhexidine-resist-ant
Klebsiella pneumoniae strain and selected transformants with chlorhexidine resistance.
A novel gene, cepA, with 903 nucleotides,
was found to be present in all
chlorhexidine-resistant clones. The predicted
amino acid sequence was compatible with a
cation efflux pump. Expression of cepA in
Escherichia coli XLOLR yielded a 32.8-kDa
protein. Retransformation using
cepA-containing plasmid conferred chlorhexidine
resistance to both E. coli XLOLR and a
chlorhexidine-sensitive K. pneumoniae strain.
We concluded that cepAis associated with
chlorhexidine resistance in K. pneumoniae.
The CepA protein may act as a cation efflux
pump to export cations such as chlorhexidine
out from bacterial cytoplasm.
Subjects
Chlorhexidine
Drug Resistance
Klebsiella pneumoniae
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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