Investigation of the disparity of chemoresistance and anti-apoptosis between colon cancer cells and colon cancer stem-like cells
Date Issued
2010
Date
2010
Author(s)
Han, Hsiang-Yu
Abstract
In recent years, the hypothesis of cancer stem-like cells (CSCs) received striking attention. This hypothesis indicated CSCs possess stem cell properties, the ability of self-renew and differentiate, and proliferate infinitely. Besides, they also have characteristics of chemotherapy-resistance and anti-apoptosis. CSCs are more difficult to eradicate and easy to cause cancer relapse because of these properties. Therefore, the mechanisms for CSCs which can escape from death after therapy merits further study.
Here, colorectal cancer cells (CRCs) were studied by investigating the characteristics of drug-resistance and anti-apoptosis within colon CSCs and non-CSCs. First, colon CSCs and non-CSCs were separated by FACS. The cytotoxicity assay showed that CSCs were more resistant to clinic chemotherapeutic drugs than non-CSCs. Moreover, the result of real-time PCR indicated that the gene expression of CSCs on certain drug resistant and anti-apoptosis was at a higher level than non-CSCs. Therefore, these findings provided practical information to design more effective therapeutic strategy for eradicating CRC.
Subjects
cancer stem-like cell
real-time PCR
drug resistance
anti-apoptosis
sphere culture
cell sorting
SDGs
Type
thesis
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