Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy
Journal
Journal of Biomedical Optics
Journal Volume
15
Journal Issue
3
Pages
36005
Date Issued
2010
Author(s)
Lo, W.
Wang, T.J.
Chen, W.-L.
Hsueh, C.-M.
Chen, S.-J.
Chen, Y.-F.
Chou, H.-C.
Lin, P.J.
Abstract
We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.
SDGs
Type
journal article