Supported Ionic Liquid for the CEC Separation of Enkephalins and Neurotensins
Date Issued
2007
Date
2007
Author(s)
Chen, Huei-Chi
DOI
zh-TW
Abstract
Two peptides enkephalins and neurotensins were carried out with supported-ionic liquid open-tubular columns. To prepare ionic liquid columns, the capillary was first silanzed with 3-(chloropropyl)- trimethoxysilane (CPTMS). Imidazole was then subjected to be bond covalently to the CPTMS in the presence of toluene or DMF as solvent. Finally imidazole was linked with 1-bromooctane to form a positively charged diakylimidazolium layer for the generation of reversed electroosmotic flow (EOF) over a pH range of 4 to 7. Using phosphate as background electrolyte (BEG) the strong ion-pair interaction between diakylimidazolium and phosphate caused the bleeding of stationary phase. With running buffer of acetate (pH 4, 100 mM)/10% (v/v) 1-propanol, [Leu]-enkephalin and [Met]-enkephalin were baseline separated within 10 min with RSD < 3.31% (n=5). With running buffer of acetate (pH 5, 10 mM)/10% (v/v) methanol, four closely related neurotensins showed three absorbed peaks within 15 min with RSD < 7.8% (n=5). In this study, the migration pattern of imidazole coated column was also compared with those of different carbon length of substituent on ionic liquid column. Because of the strong hydrogen bond interaction, the peaks of imidazole coated column showed more tailing. For C8 or C12 of substituent of ionic liquid, the hydrophobic force and hydrogen bond interaction were stronger, and the column efficiency was better than the short carbon chain length (C4) .
Subjects
離子液體
毛細管電層析
ionic liquid
CEC
Type
thesis
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