Morphopathological and Immunohistochemical Studies on Histogenesis of Canine Hemangiopericytoma
Date Issued
2007
Date
2007
Author(s)
Shu, Che-Ming
DOI
zh-TW
Abstract
Canine hemangiopericytoma (CHP) is a soft tissue tumor of skin, often found in middle to old age of dogs, and arises in the subcutis of joint and limbs. CHP with morphopathological, immunohistochemical and electronic microscopical studies, suggests this is pericytic origin. The actual histogenesis is uncertain, and classified into unclassified tumor of soft tissue of skin in WHO. Human hemangiopericytoma (HP) and human myopericytoma (MP) respectively classified as fibroblastic/myofibroblastic and pericytic tumor by WHO. A total of 18 CHP were used in this study and 18 HP were also collected to compare with CHP. The peak incidence of CHP was between 7 to 12 years (67 %; 12/18) of age, and sex predilection was male (67 %; 12/18). Most common breed affected was mongrel (33 %; 6/18). Most of the cases were located in the subcutis of limb, especially in joint and foot (56 %; 10/18). Histopathologically, CHP revealed perivascular whorls of fusiform and ovoid tumor cells, interlacing and storiform pattern, and concentric arrangement with capillary and medium to small blood vessels, and similar to MP. But, HP characterized small ovoid, round to spindle tumor cells arranged in solid to sheets and surrounding thin walled, branching, stag-horn pattern of dilated blood vessels. The IHC profile of CHP showed vimentin (100 %; 18/18), NSE (100 %; 18/18), S-100 (78 %; 14/18), desmin (67 %; 12/18), α-SMA (61 %; 11/18), HHF-35 (56 %; 10/18), neurofilament (33 %; 6/18)、Factor VIII (28 %; 5/18)、Collagen IV (6 %; 1/18)、laminin (0 %; 0/18) and NGFR (0 %; 0/18). CHP showed vimentin (100 %; 18/18)、Factor VIII (78 %; 4/18), HHF-35 (17 %; 3/18), α-SMA (11 %; 2/18), desmin (0%; 0/18), Collagen IV (0 %; 0/18) and laminin (0%; 0/18). IHC profiles of CHP resembled in MP that both they expressed in muscle marker. Ultrastructurally, the tumor cells were arranged in compact to loose with irregular cell processes, basal lamina, dilated rough endoplasmic reticulum, and variable sized pinocytes and microfilaments in cytoplasm. In conclusion, features of morohopathology, IHC profile and ultrastructural findings reveal obvious divergence between CHP and HP and resemble MP, so we suggest that histogenesis of CHP is pericyte with myoid differentiation.
Subjects
犬血管周圍細胞瘤
人類
血管周圍細胞瘤
免疫組織化學
Canine hemangiopericytoma
Human hemangiopericytoma
Immunohistochemistry
Type
thesis
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