Aminophenylboronic acid polymer nanoparticles for quantitation of glucose and for insulin release
Journal
Analytical and Bioanalytical Chemistry
Journal Volume
408
Journal Issue
24
Pages
6557-6565
Date Issued
2016
Author(s)
Abstract
We have developed a simple route for the preparation of aminophenylboronic acid polymer nanoparticles (APB PNs) from 3-aminophenylboronic acid and formaldehyde under alkaline conditions according to an extended St?ber method. Insulin and R6G have been selected to prepare functional insulin-APB PNs and R6G-APB PNs, respectively. During the formation of APB PNs, the representative molecules are embedded inside the APB PNs. Through specific binding of glucose with boronic acid moieties on the R6G-APB PNs and insulin-APB PNs, glucose induces expansion of the APB PNs, leading to release of R6G and insulin molecules, respectively. As a result of release of R6G molecules, the fluorescence intensity of R6G-APB PN solution increases, allowing quantitation of glucose in PBS solutions (10 mM, pH 7.4) with a linear range over 0–10 mM. Release of insulin from insulin-APB PNs is significant and rapid when the glucose concentration is higher than 7 mM. Having advantages of low cost, simple preparation, biocompatibility, and continuous response to glucose, the insulin-APB PNs hold great potential as an alternative for treating diabetic patients. [Figure not available: see fulltext.] ? 2016, Springer-Verlag Berlin Heidelberg.
Subjects
Boronic acid; Glucose detection; Insulin release; Polymeric nanoparticles
SDGs
Other Subjects
Alkalinity; Biocompatibility; Glucose; Molecules; Nanoparticles; Polymers; 3-Aminophenylboronic acid; Aminophenylboronic acid; Boronic acid; Fluorescence intensities; Glucose concentration; Glucose detection; Insulin release; Polymeric nanoparticles; Insulin; 3-aminobenzeneboronic acid; boronic acid derivative; fluorescent dye; insulin; nanoparticle; rhodamine; rhodamine 6G; adult; analysis; chemistry; female; glucose blood level; human; procedures; spectrofluorometry; ultrastructure; Adult; Blood Glucose; Boronic Acids; Female; Fluorescent Dyes; Humans; Insulin; Nanoparticles; Rhodamines; Spectrometry, Fluorescence
Type
journal article