White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly
Resource
Proceedings of the National Academy of Sciences 105 (52): 20758-20763
Journal
Proceedings of the National Academy of Sciences
Journal Volume
105
Journal Issue
52
Pages
20758-20763
Date Issued
2008
Date
2008
Author(s)
Wang, Hao-Ching
Wang, Han-Ching
Ko, Tzu-Ping
Lee, Yu-May
Leu, Jiann-Horng
Ho, Chun-Han
Lo, Chu-Fang
Wang, Andrew H.-J.
Abstract
White spot syndrome virus (WSSV) is a large (?300 kbp), double-stranded DNA eukaryotic virus that has caused serious disease in crustaceans worldwide. ICP11 is the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance in WSSV infection; but until now, its function has remained obscure. We show here that ICP11 acts as a DNA mimic. In crystal, ICP11 formed a polymer of dimers with 2 rows of negatively charged spots that approximated the duplex arrangement of the phosphate groups in DNA. Functionally, ICP11 prevented DNA from binding to histone proteins H2A, H2B, H3, and H2A.x, and in hemocytes from WSSV-infected shrimp, ICP11 colocalized with histone H3 and activated-H2A.x. These observations together suggest that ICP11 might interfere with nucleosome assembly and prevent H2A.x from fulfilling its critical function of repairing DNA double strand breaks. Therefore, ICP11 possesses a functionality that is unique among the handful of presently known DNA mimic proteins. ? 2008 by The National Academy of Sciences of the USA.
Subjects
Apoptosis; Crystal structure; DNase enhancer; Shrimp aquaculture
SDGs
Other Subjects
amino acid derivative; deoxyribonuclease; double stranded DNA; histone H2A; histone H2B; histone H3; polymer; protein icp11; unclassified drug; virus DNA; virus protein; article; cell structure; controlled study; DNA binding; human; human cell; immunofluorescence test; in vivo study; nucleosome; priority journal; protein function; protein localization; White spot syndrome virus; Animals; Crystallography, X-Ray; Dimerization; DNA; Hemocytes; Histones; Nucleosomes; Penaeidae; Protein Binding; Protein Structure, Quaternary; Viral Proteins; White spot syndrome virus 1; Crustacea; Decapoda (Crustacea); Eukaryota; Shrimp white spot syndrome virus
Type
journal article
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