Detection and analysis of virus-infected cells in cats with feline infectious peritonitis
Date Issued
2009
Date
2009
Author(s)
Wu, Cheng-Wei
Abstract
Feline infectious peritonitis (FIP) is an immune-mediated disease manifesting clinically as effusive or non-effusive form. The causative agents, feline coronaviruses (FCoVs), are serologically classified into type I and II. In order to understand the differences in the distribution pattern of FCoV-infected cells within the lesions between effusive and non-effusive FIP, cats presented to the Veterinary Hospital at National Taiwan University with the final diagnosis of FIP were selected. Tissue samples from five typical non-effusive cases and eight typical effusive cases were subjected to immunohistochemistry staining. FCoV-infected cells were found in nearly all kinds of the examined organ samples. The FCoV-positive rates of kidney (80%) and brain (100%) in non-effusive FIP were higher than those of effusive FIP (37.5% and 40%, respectively). As comparing to effusive cases, a tendency that the lesions associated with FCoV were more abundant and severe in non-effusive ones was noted. To correlate different type of FCoVs with the development of FIP, in situ amplification of FCoV nucleic acid in affected tissues was attempted using type-specific primers via direct in situ RT-PCR. As the process of digestion might greatly influence the test results, optimization of the assay condition was first established. For F96-156, a FIP case that infected with type I and II FCoVs, incubation with 50 μg/mL of proteinase K at 37℃ for one hour in liver sections, and 2 mg/mL of pepsin at room temperature for four hours in lung sections were found to be optimal. Under these optimized conditions, type II FCoV-infected cells were detected in both liver and lung.
Subjects
feline infectious peritonitis
feline coronavirus
dry form
non-effusive form
wet form
effusive form
direct in situ reverse transcription polymerase chain reaction
direct in situ RT-PCR
immunohistochemistry
IHC
typing
Type
thesis
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