Investigation of P1/HC?pro?mediated ABA/calcium signaling responses via gene silencing through high? and low? throughput RNA?seq approaches
Journal
Viruses
Journal Volume
13
Journal Issue
12
Date Issued
2021
Author(s)
Chiu Y.-H
Hung Y.-L
Wang H.-P
Wei W.-L
Shang Q.-W
Pham T.H
Huang C.-K
Pan Z.-J
Abstract
The P1/HC?Pro viral suppressor of potyvirus suppresses posttranscriptional gene silencing (PTGS). The fusion protein of P1/HC?Pro can be cleaved into P1 and HC?Pro through the P1 self-cleavage activity, and P1 is necessary and sufficient to enhance PTGS suppression of HC?Pro. To address the modulation of gene regulatory relationships induced by turnip mosaic virus (TuMV) P1/HC?Pro (P1/HC?ProTu), a comparative transcriptome analysis of three types of transgenic plants (P1Tu, HC?ProTu, and P1/HC?ProTu) were conducted using both high?throughput (HTP) and low-throughput (LTP) RNA?Seq strategies. The results showed that P1/HC?ProTu disturbed the endogenous abscisic acid (ABA) accumulation and genes in the signaling pathway. Additionally, the inte-grated responses of stress?related genes, in particular to drought stress, cold stress, senescence, and stomatal dynamics, altered the expressions by the ABA/calcium signaling. Crosstalk among the ABA, jasmonic acid, and salicylic acid pathways might simultaneously modulate the stress responses triggered by P1/HC?ProTu . Furthermore, the LTP network analysis revealed crucial genes in common with those identified by the HTP network in this study, demonstrating the effectiveness of the miniaturization of the HTP profile. Overall, our findings indicate that P1/HC?ProTu?mediated suppression in RNA silencing altered the ABA/calcium signaling and a wide range of stress responses. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
ABA signaling
Calcium signaling
HTP?Seq
LTP?Seq
P1/HC?ProTu
Stress response
E3 ubiquitin protein ligase RDUF1
messenger RNA
P1 HC pro
protein
protein kinase
suppressor factor
transcriptome
unclassified drug
abiotic stress
abscisic acid signaling
ago1 27
Arabidopsis thaliana
arabidopsis thaliana ABA related RING type E3 ligase
arabidopsis toxicos en levadura 27
Article
biotic stress
calcium signaling
cytochrome P450 family 707 subfamily A polypeptide 3
differential gene expression
DNA library
DTX50
electrospray
gene
gene silencing
germination
high throughput sequencing
homeostasis
immune response
liquid chromatography
mass spectrometry
MEE31
molecular genetics
mutant
nonhuman
plant gene
plant growth
plant seed
plastid movement impaired1
PUB22
PUB23
real time polymerase chain reaction
RNA extraction
RNA sequencing
sensitivity analysis
signal transduction
whole transcriptome sequencing
Type
journal article