Selection of aptamers for AMACR detection from DNA libraries with different primers
Journal
Rsc Advances
Journal Volume
34
Pages
19067-190748
Date Issued
2018
Author(s)
Abstract
In this work, we investigated aptamer selection against alpha-methylacyl-CoA racemase (AMACR) with three DNA libraries bearing different primers. Because of increased selection diversity, aptamers with varied structural properties, such as pre-folded, induced-folding, high and low primer-dependent conformations, were discovered. From the selection results, a dimeric aptamer was constructed and capable of detecting over-expression of AMACR in prostate cancer cell lines. In summary, this study demonstrates a library-based approach to obtain aptamers with different binding properties and provides distinct aptamers for flexible design of AMACR detection. ? The Royal Society of Chemistry 2018.
SDGs
Other Subjects
Binding energy; Cell culture; Diseases; Aptamers; Binding properties; DNA library; Flexible designs; Methylacyl-CoA racemase; Over-expression; Prostate cancer cells; Selection diversity; Libraries
Type
journal article