The regulation of ABI5 expression by ABF3 in response to salt and ABA in Arabidopsis thaliana
Date Issued
2016
Date
2016
Author(s)
Chang, Hui-Chun
Abstract
Basic region/leucine zippers (bZIPs) are transcription factors (TFs) encoded by a big gene family in plants. Previous studies showed that bZIPs participate in many abiotic stress responses. bZIPs can regulate many down-stream genes, which could response against the environment stresses. However, the transcriptional network and the transcription mechanism in plants remained unclear. 14-3-3 proteins are scaffold proteins, which can result in activation/deactivation of enzymes, alteration of the translocation into/out of organelles like the nucleus, mitochondria and chloroplasts, prevention or stimulation of proteolytic breakdown of protein. The gene expression of ABF3 and ABI5 are induced by salt. In order to investigate the relationship between ABF3, a bZIP TF, and 14-3-3 protein in the transcriptional network in Arabidopsis, This research carried by protoplast transactivation assay using luciferase as a reporter. The results showed that the expression of both 14-3-3 and ABF3 can up-regulate ABI5 gene expression. Chromatin immunoprecipitation (ChIP) analysis results showed that ABF3 binds to the promoter of ABI5. In vitro kinase assay showed that calcium dependent protein kinase can phosphorylate ABF3. Thr-128, Ser-134 and Thr-451 phosphorylation sites were identified. In conclusion, 14-3-3 and ABF3 together regulate the transcription level of down-stream ABI5 gene. Whether this regulation pathway is involved in salt stress response requires further studies.
Subjects
bZIP
14-3-3
bimolecular fluorescence complementation
salt stress
phosphorylation
Type
thesis
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