Infrequent hMSH2 mutations in sporadic gastric adenocarcinoma with microsatellite instability
Journal
Cancer Letters
Journal Volume
112
Journal Issue
2
Pages
161-166
Date Issued
1997
Author(s)
JIN-CHUAN SHEU
Lee W.-J.
Wang T.-H.
Lin J.-T.
Abstract
The status of genetic instability was determined with seven microsatellite markers from 40 patients with primary gastric adenocarcinoma. For those cases with microsatellite instability, alterations of hMSH2 were further investigated by direct sequencing of reverse transcription-polymerase chain reaction products. Twelve (30%) of 40 patients were found to have microsatellite instability, Among them, one patient (1/6, 16.7%) was early gastric cancer and 11 (11/34, 32.4%) were advanced gastric cancer. There were seven patients with diffuse type (7/18, 38.7%), while five (5/22, 22.7%) were intestinal type tumors. The entire coding region of the hMSH2 gene in these 12 affected individuals was amplified and sequenced. Only a 41-year-old female patient with diffuse type advanced gastric cancer showed a GCT to TCT missense mutation at codon 207 with predicted protein change from alanine to serine. Our results indicate that genetic instability plays an important role in gastric tumorigenesis and alterations of the hMSH2 gene are related to only a small portion of sporadic gastric adenocarcinoma with microsatellite instability.
SDGs
Other Subjects
alanine; microsatellite dna; serine; tumor marker; adult; advanced cancer; aged; amino acid substitution; article; clinical article; codon; early cancer; gene amplification; gene mutation; genetic stability; human; human tissue; missense mutation; priority journal; reverse transcription polymerase chain reaction; stomach adenocarcinoma; stomach carcinogenesis; Adenocarcinoma; Adult; Aged; Aged, 80 and over; DNA Replication; DNA, Neoplasm; DNA, Satellite; DNA-Binding Proteins; Electrophoresis; Female; Genetic Markers; Humans; Male; Middle Aged; Mutation; MutS Homolog 2 Protein; Polymerase Chain Reaction; Proto-Oncogene Proteins; Stomach Neoplasms; Transcription, Genetic
Type
journal article