Characterization of corneal damage from Pseudomonas aeruginosa infection by the use of multiphoton microscopy
Journal
Applied Physics Letters
Journal Volume
97
Journal Issue
18
Date Issued
2010
Author(s)
Abstract
Using multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy, we investigate the morphology and the structure of the corneal epithelium and stroma collagen of bovine cornea following injection of Pseudomonas aeruginosa. We found that corneal epithelial cells are damaged and stromal collagen becoming increasingly autofluorescent with time. We also characterized infected cornea cultured for 0, 6, 12, and 24 h by quantitative ratiometric MAF to SHG index (MAFSI) analysis. MAFSI results show that the destruction of the stromal collagen corresponds to a decrease in SHG intensity and increase of MAF signal with time. © 2010 American Institute of Physics.
Type
journal article