Behavior and Functionality of Neurons Cultured on EVAL Membranes Modified With Lysine and 1,4-Diaminobutane
Date Issued
2009
Date
2009
Author(s)
Lin, Chun-Hung
Abstract
We prepare poly(ethylene-co-vinyl alcohol)(EVAL) membranes by the dry processing ,and then grafted methacryloyl chloride (MAC)on the hydroxyl group of these membrane surface by the way of chemical modification. Furthermore, we grafted different diamines, lysine and modified lysine with 1,4-diaminobutane by EDC/NHS method on them. We cultured the cerebellar granule neurons of the Wistar rat on the modified membranes and compared with the relative activity by MTT assays. In addition, the cell morphology and behavior was observed by optical microscope and time-lapse. The functional structures of cerebellar granule neurons cultured on different membranes were observed by fluorescence microscope. We also used Western blot and glutamate release to mesure the protein expression and neural transmission. Overall, the modification of the EVAL with lysine modified 1, 4-diaminobutane ( E-Lys-14) have better relative activity, higher protein expression level and longer culture duration than coating PDL. E-Lys-14 may be the batter substrate to substitude coating PDL to culture neurons.
Subjects
EVAL
cerebellar granule cells
methacryloyl Chloride
EDC
PDL
1,4-diaminobutane
lysine
Type
thesis
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