Methicillin-Resistant Staphlococcus aureus(MRSA)Isolates from a Swine Meat Market and a Slaughterhouse in Northern Taiwan
Date Issued
2011
Date
2011
Author(s)
Lo, Ya-Ping
Abstract
Increasing prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has been described in many countries recently, such as ST398 in Europe and North America, while ST9 has been detected from swine and workers in Asia areas, such as China and Malaysia. In total, 299 swine nasal samples were collected from a meat market and a slaughterhouse in this study. Molecular techniques including multilocus sequence typing (MLST), pulse-field gel electrophoresis (PFGE), and staphylococcal chromosomal cassette mec (SCCmec) typing were applied to characterize MRSAs. The prevalence of MRSA was 42.5% (127/299). All isolates were PVL negative, and were assigned as MLST ST9. According to PFGE results, a total of 166 MRSAs were recovered. Five clusters of pulso-types were grouped with similarity of all higher than 71%, except a SmaI non-typeable strain. Thirty-six pigs (28.3%) harbored more than one MRSA strain which shared same PFGE results. The majority of the isolates (160) belonged to SCCmec type V, and 4 others were type IV, and the rest two were SCCmec non-typeable. Among spa typing, more than 90% of isolates were t899, the rest were t1939, t2922, t4132, t4358, a novel type- t7616, and a non-spa-typeable isolate. In the result of agar breakpoint method, all MRSAs were susceptible to vancomycin and resistant to clindamycin. There were more than 80% of isolates resistant to gentamicin (99.4%), tetracycline (99.4%), ciprofloxacin (98.8%), trimethoprim-sulfamethoxazole (90.0%), erythromycin (83.7%), and less than 50% to chloramphenicol (42.2%) and rifampin (13.3%). Among micro-broth dilution method, the percentage of chloramphenicol resistance was 78.9%, and rifampin resistance was 1.8%.In conclusions, high prevalence of LA-MRSA ST9 was found and the isolates were the same as those reported in some Asia countries. High prevalence of MRSA with multidrug resistance in livestock deserves public health concerns for workers in swine-associated environments.
Subjects
meat market
MRSA
slaughterhouse
swine
SDGs
Type
thesis
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