網膜色素上皮層對鞏膜細胞生長之控制
Date Issued
1999
Date
1999
Author(s)
施永豐
DOI
882314B002373
Abstract
Atropine was found to be effective in arresting the progression of myopia. However, the actual mechanism is still unclear. Thus, we tried to investigate the in viv; effect of atropine on the proliferation of scieral chondrocytes in chicks of font-deprivation myopia. Twenty chicks were equally divided into 4 groups which included intravitretd injection of normal saline (IVN), IVN with goggling (IVNG), intravitreal injection of atropine (%) (IV A), and WA with goggling (IVAG) groups. Intravenous injection of bromodeoxyuridine (BrdU) (30 mg/kg) from subaxillary vein was performed 2 hours before being sacrificed. The eyeballs were then fixed in 10% neutral-buffered formalin at
4¢XC. Standard BrdU immunohistochemical staining was pcrformei. The BrdU labeling index was obtained from the average of positive labelings of BrdU in scleral chondrocytes for every 100 counting cells in posterior poles and anterior scleral margins by two experienced technicians. The BrdU index on the anterior scleral margin of the IVAG group was less than that of the PING group. The index on the anterior scleral margin of the IVNG group was higher than the IVN group. Although the index on the posterior poles of the IVNG group was also higher ft an the IVN group, it was statistically not different. Also, no statistical difference was found between IVN and IVA on the anterior scleral margins or posterior poles. The index was significantly dif[erent on the anterior scleral margins, but not on the posterior pole among each group. Therefore intravitrcal injection of atroptne could inhibit the proliferation of chondrocytes on the anterior margins of sclera, but not the posterior poles in form-deprivation myopia.
Publisher
臺北市：國立臺灣大學醫學院眼科
Type
journal article
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