Cellular Extract Preparation for Superoxide Dismutase (SOD) Activity Assay
Journal
Bio-protocol
Journal Volume
3
Journal Issue
13
Pages
e811
Date Issued
2013
Author(s)
Abstract
Superoxide dismutase (SOD) acts as a primary defence against reactive oxygen species (ROS) by converting O2- to O2 and H2O2. Members of this enzyme family include CuZnSOD, MnSOD and FeSOD. Most eukaryotes harbor CuZnSOD and MnSOD, and FeSOD is found in plants and prokaryotes. This protocol is to demonstrate how to prepare the cellular extract for the identification and characterization of SODs in planta.
SDGs
Publisher
Bio-protocol LLC.
Type
journal article