Cell Capture and Separation Using Dielectrophoresis and Microfluidics
Date Issued
2014
Date
2014
Author(s)
Chang, Ya-Chu
Abstract
A device for selective particle capture and separation using microfluidics and dielectrophoresis was developed in the thesis. The device is a micro channel with grooves built on its ceiling. Electrodes are built on the bottom surface of the device on both the upstream and downstream sides of the groove. Negative dielectrophoretic force is exerted on the test particle when it passes through the electrode gap. Particles with selected Kr (the real part of the Clausius-Mossotti factor) will be pushed into the channel and are thus captured. Other particles with different Kr will be carried downstream, and thus particles with different dielectric properties are separated. The device was reported in the literature, but here we propose a modify version of the device such that the two particles to be separated can be counted, and thus the capture rate and purity of separation can be evaluated accurately. Therefore, the modified device proposed here is capable of determining suitable operation parameters for a given separation, which is necessary for a separating device based on the intrinsic physical properties of particles. As for the separation of polystyrene particles and cancer cells (including the lung cancer cells CL1-0 and CL1-5, and the colorectal cancer cells Colo205), 100% can be separated under a frequency 1 MHz and a background flow rate below 40ul/hr. 80% separation can be achieved for the mixture of CL1-0 and Colo205 in a medium with conductivity 0.11S/m, at a flow rate 30ul/hr and frequency 70kHz. The geometric and operation parameters of the device were also studied numerically and experimentally.
Subjects
Microfluidics device
Dielectrophoresis
Particle capture and separation
SDGs
Type
thesis
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