The characteristics of MHC expression and cytokine profile of spontaneous canine tumors
Date Issued
2007
Date
2007
Author(s)
Chang, Li-Ti
DOI
zh-TW
Abstract
There is lack of detailed MHC (Major histocompatibility complex) and cytokine profile data available in canine spontaneous tumors. However, this data is essential in a gene therapy application. 62 cancer dog patients were mostly collected from animal hospital in Taipei. Single cell suspensions were prepared. The flow cytometry was used to analyze the MHC I, II of tumor cells, subpopulations of TIL (Tumor infiltrating lymphocyte), PBL (Peripheral blood lymphocyte) including MHC I, II, and CD3, 4, 8, 21. In addition, tumor cells cytokines, such as IL-2, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, TNF-α, TGF-β, IFN-γ were studied by real-time RT PCR. These data were compared with the case history and their prognosis by Biomarker Patterns Software and we found that the cutoff value of MHC I expression in MGT (Mammary gland tumor) and non-MGT was 72.58%. Lower than 72.58% was MGT, and higher would be non-MGT. The results from 36 MGT and 15 non-MGT were collected. For MGT, 33 of 36 cases (91%) the MHC I were low in density (lower than 72.58%). 2 of 3 (67%) benign tumor and 31/33 (94%) malignant tumors expressed low MHC I in density. Therefore, among most spontaneous tumors, MGT tent to expresses low MHC I and low MHC I expression may have correlation with tumor malignancy. Data below are cytokine mRNA from MGT, benign MGT, and tubulopapillary carcinoma in malignant MGT compared with healthy mammary gland (HMG). IL-8 in MGT was significantly higher, while IL-10, IL-12, IL-15 in benign MGT and IL-12 in tubulopapillary carcinoma are significantly lower than HMG. Moreover, malignant MGT express significantly higher IL-8 and IL-13 mRNA level than benign MGT. Although IL-8 is significantly higher in MGT than HMG, only malignant MGT and especially simple carcinoma is significantly higher than mammary gland. Other subtypes of MGT has no significance compared to HMG. IL-8 may have positive correlation with angiogenesis. In addition, MGT tended to have more non-T, non-B cells than healthy dogs in the PBL. In cell surface marker, low MHC II in tumor cell, high CD4 and CD21 in TIL, high MHC II, non-CD4,8,21 and low CD21 in PBL has statistically significance in prognosis.
From data above, MGT tend to express lower percentage of MHC I among spontaneous tumors collected in this study. In addition, low MHC I may be correlated with tumor malignancy. In addition, cell surface marker and mRNA expression profile in PBL and tumor cell may help us understand tumorigenesis mechanism, and the correlations with prognosis, which is useful for the study of canine cancer immunotherapy.
Subjects
自然發生腫瘤
主要組織相容複合物
腫瘤浸潤淋巴球
週邊血液淋巴球
細胞激素
概述
血管新生
預後
spontaneous tumor
major histocompatibility complex (MHC)
tumor infiltrating lymphocyte (TIL)
peripheral blood lymphocytes (PBL)
cytokine
profile
angiogenesis
prognosis
SDGs
Type
thesis
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