2,3,5,4'-tetrahydroxystilbene-2-O-D-glucoside suppresses human colorectal cancer cell metastasis through inhibiting NF-B activation
Journal
International Journal of Oncology
Journal Volume
49
Journal Issue
2
Pages
629-638
Date Issued
2016
Author(s)
Abstract
2,3,5,4'-tetrahydroxystilbene-2-O-D-glucoside (THSG), a major component of Polygonum multiflorum Thunb (He-Shou-Wu), has been reported to exhibit antioxidant and anti-inflammatory effects. However, its anti-metastatic effect against colorectal cancer is still unclear. In this study, cell migration, invasion and adhesion abilities as well as metastasis-associated protein and NF-B pathway signaling factor expression were analyzed after treating HT-29 cells with THSG. According to the results, the migration and invasiveness of HT-29 cells were reduced after treatment with 5 or 10 mM THSG (p<0.05). Additionally, the levels of matrix metalloproteinase-2 (MMP-2) and phosphorylated VE-cadherin in HT-29 cells were reduced and the transepithelial electrical resistance (TEER) of EA.hy926 endothelial cell monolayers was increased after incubation in THSG for 24 h (p<0.05). Cell adhesion ability and the E-selectin and intercellular adhesion molecule-1 (ICAM-1) protein levels were reduced when EA.hy926 endothelial cells were treated with THSG (p<0.05). In addition, the cytoplasmic phosphorylation of IB, the nuclear p65 level and the DNA-binding activity of NF-B were reduced after treating HT-29 or EA.hy926 cells with 5 or 10 mM THSG (p<0.05). These results suggest that THSG inhibits HT-29 cell metastasis by suppressing cell migration, invasion and adhesion. Furthermore, THSG inhibits metastasis-associated protein expression by suppressing NF-B pathway activation.
Subjects
2,3,5,4'-tetrahydroxystilbene-2-O-D-glucoside; Adhesion; Human colorectal cancer cells; Invasion; Metastasis; Migration; NF-B
SDGs
Other Subjects
2,3,5,4' tetrahydroxystilbene 2 o beta dextro glucoside; antimetastatic agent; chemical compound; endothelial leukocyte adhesion molecule 1; gelatinase A; gelatinase B; I kappa B; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; unclassified drug; vascular endothelial cadherin; 2,3,5,4'-tetrahydroxystilbene-2-O-glucoside; glucoside; immunoglobulin enhancer binding protein; stilbene derivative; Article; cell adhesion; cell invasion; cell invasion assay; cell migration; cell viability; colorectal cancer; controlled study; DNA binding; DNA binding assay; electric resistance; endothelium cell; fluorescence microscopy; HT 29 cell line; human; human cell; immunoblotting; metastasis inhibition; priority journal; protein expression; protein phosphorylation; transcription initiation; transepithelial electrical resistance; wound healing; antagonists and inhibitors; cell motion; Colorectal Neoplasms; drug effects; HT-29 cell line; metabolism; metastasis; pathology; signal transduction; Cell Movement; Colorectal Neoplasms; Glucosides; HT29 Cells; Humans; Neoplasm Metastasis; NF-kappa B; Signal Transduction; Stilbenes
Publisher
Spandidos Publications
Type
journal article