Production of glucosinolates by tissue culture of Brassica oleracea var. italica
Date Issued
2012
Date
2012
Author(s)
Li, Jia-Ling
Abstract
Brassicaceae family is popular vegetable around the world, and it contains secondary metabolites-glucosinolates, which are potent source of protective chemicals against cancer. Glucosinolates can induce the phase II enzyme system thus inhibiting the growth of tumor.
The purpose of our efforts was to produce glucosinolates by plant tissue culture, and we chose broccoli, one of the important vegetables in Brassicaceae family, as material. The experiment was divided into three stages. Firstly, we tried to find out an optimal medium to induce broccoli to form callus. After investigating different kinds and concentrations of plant hormones, we found that addition of 1 g/ mL 2,4-D and 0.5 g/mL BA in the MS medium could obtain the best result. The percentage of health callus was above 80. In the second stage, shaker flask was used to cultivate broccoli callus in suspension culture. It was found that the glucobrassicin increased after 7 days of cultivation. Finally, fermentor was used to cultivate broccoli callus. We found that glucosinolates were mostly in the callus, and only a little released to the medium. The content of glucosinolates in callus cultivated by air lift fermentor with 5 % CO2 was the highest. However, the callus with the highest antioxidant activity was obtained in air lift fermentor without CO2, and it also had the highest phenolics content. The HPLC profile revealed that the major glucosinolates appeared at retention time 27 min, and MS analysis indicated that the compound is 4-Methoxyglucobrassicin.
Subjects
broccoli
callus
secondary metabolites
fermentor
glucosinolates
SDGs
Type
thesis
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