Early Detection of Lewis Lung Carcinoma Tumor Control by Irradiation Using Diffusion-Weighted and Dynamic Contrast-Enhanced MRI
Journal
PLoS ONE
Journal Volume
8
Journal Issue
5
Date Issued
2013
Author(s)
Lu, Y.-C.
Cho, K.-H.
Wu, J.-K.
Wu, C.-J.
Abstract
Purpose:To investigate the correlation between diffusion-weighted (DW) and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) derived parameters and radioresponsiveness of Lewis lung carcinoma (LLC) tumor.Materials and Methods:LLC tumor growth in C57BL/6 mouse limb was used for the experiment. The tumors were irradiated with 10 Gy×5, or 30 Gy×2 vs. sham irradiation. Fourteen tumors were subjected to DW-MRI and DCE-MRI pre-radiotherapy and weekly imaging after radiotherapy. The temporal changes in apparent diffusion coefficient (ADC) and DCE-MRI derived parameters (Ktrans, kep, ve, and vp) were correlated with tumor size, and were histologically compared with CD31 staining of resected tumors.Results:The 10 Gy×5 dose inhibited tumor growth for a week, while 30 Gy×2 controlled tumor growth for a 3-week observation period. One week after radiotherapy (week 2), irradiated tumors showed significantly higher values of ADC than untreated ones (10 Gy×5, p = 0.004; 30 Gy×2, p = 0.01). Significantly higher values of ve were shown earlier by 30 Gy×2 vs. sham (p = 0.01) and 10 Gy×5 vs. sham irradiation (p = 0.05). Sustained higher ve from 10 Gy×5 compared to sham irradiated tumors was evident at week 3 (p = 0.016) and week 4 (p = 0.046). A 13.8% early increase in ADC for 30 Gy×2 tumor group (p = 0.002) and a 16.5% increase for 10 Gy×5 group were noted (p = 0.01) vs. sham irradiation (which showed a 2.2% decrease). No differences were found for Ktrans, kep, or vp. Both radiotherapy groups demonstrated significant reduction in microvessel counts.Conclusion:Early increase in ADC and ve correlated with tumor control by irradiation. ? 2013 Cheng et al.
SDGs
Other Subjects
CD31 antigen; animal experiment; animal model; article; cancer control; cancer inhibition; cancer radiotherapy; computer assisted tomography; contrast enhancement; controlled study; diffusion coefficient; diffusion weighted imaging; dynamic contrast enhanced magnetic resonance imaging; early diagnosis; histopathology; Lewis carcinoma; male; mouse; nonhuman; nuclear magnetic resonance imaging; positron emission tomography; radiation dose; tumor growth; tumor volume; Animals; Carcinoma, Lewis Lung; Contrast Media; Diffusion Magnetic Resonance Imaging; Early Detection of Cancer; Male; Mice; Mice, Inbred C57BL; Tumor Burden
Type
journal article