Borapetoside C from Tinospora crispa improves insulin sensitivity in diabetic mice
Journal
Phytomedicine
Journal Volume
19
Journal Issue
8月9日
Pages
719-724
Date Issued
2012
Author(s)
Abstract
Diabetes mellitus (DM) often leads to disability from vascular complications and neurological complications. Tinospora crispa has been widely used in Asia and Africa as a remedy for diabetes and other diseases. In this study, we investigated the hypoglycemic actions of borapetoside C isolated from T. crispa, and the mechanisms underlying its actions. Acute treatment with borapetoside C (5 mg/kg, i.p.) attenuated the elevated plasma glucose induced by oral glucose in normal and type 2 DM (T2DM) mice. Compared to the effect of injected insulin (0.5 IU/kg), borapetoside C caused a more prominent increase of glycogen content in skeletal muscle of T2DM mice, but a less increase in type 1 DM (T1DM) mice. Combined treatment of a low dose borapetoside C (0.1 mg/kg, i.p.) plus insulin enhanced insulin-induced lowering of the plasma glucose level and insulin-induced increase of muscle glycogen content. Continuous treatment with 5 mg/kg borapetoside C (twice daily) for 7 days increased phosphorylation of insulin receptor (IR) and protein kinase B (Akt) as well as the expression of glucose transporter-2 (GLUT2) in T1DM mice. Combined treatment of a low dose borapetoside C (0.1 mg/kg, twice daily) plus insulin for 7 days enhanced insulin-induced IR and Akt phosphorylation and GLUT2 expression in the liver of T1DM mice. This study proved that borapetoside C can increase glucose utilization, delayed the development of insulin resistance and enhanced insulin sensitivity. The activation of IR-Akt-GLUT2 expression and the enhancement of insulin sensitivity may contribute to the hypoglycemic action of borapetoside C in diabetic mice. ? 2012 Elsevier GmbH. All rights reserved.
SDGs
Other Subjects
borapetoside C; glucose; glucose transporter 2; glycogen; human insulin; insulin; insulin receptor; plant extract; protein kinase B; unclassified drug; animal model; animal tissue; antidiabetic activity; article; controlled study; diabetes mellitus; drug isolation; drug mechanism; glucose blood level; glycogen muscle level; insulin dependent diabetes mellitus; insulin resistance; insulin sensitivity; liver level; low drug dose; male; mouse; non insulin dependent diabetes mellitus; nonhuman; oral glucose tolerance test; priority journal; protein expression; protein phosphorylation; skeletal muscle; Tinospora; Tinospora crispa; Animals; Blood Glucose; Diabetes Mellitus, Type 2; Diterpenes; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Drug Therapy, Combination; Glucose Tolerance Test; Glucose Transporter Type 2; Glycogen; Hypoglycemic Agents; Insulin; Insulin Resistance; Liver; Male; Mice; Mice, Inbred ICR; Muscle, Skeletal; Phosphorylation; Plants, Medicinal; Proto-Oncogene Proteins c-akt; Receptor, Insulin; Signal Transduction; Tinospora; Mus; Tinospora crispa
Type
journal article