Diffusion tensor MRI of myocardial fibers and sheets: Correspondence with visible cut-face texture
Resource
POWDER TECHNOLOGY 131 (2-3): 212-222 APR 23 2003
Journal
POWDER TECHNOLOGY
Journal Volume
131
Journal Issue
23
Pages
-
Date Issued
2003
Date
2003
DOI
246246/2006111501275429
Abstract
Microsporidian isolates from five lepidopteran pests—Spodoptera litura, Spodoptera exigua, Helicoverpa armigera, Plutella xylostella,
and Pieris spp.—were compared by spore morphology, infectivity to S. litura, Western-blot bandingpatterns, the sequences
of small subunit rRNA gene (SSUrRNA sequence), and random amplified polymorphic DNA polymerase chain reaction (RAPDPCR).
All the isolates could infect experimentally and multiply in the larvae of S. litura. The S. exigua isolate showed the highest
virulence to the larvae of S. litura while the P. xylostella isolate showed the lowest. No significant differences either in spore
morphology or in SSUrRNA sequences of these isolates were found. The SSUrRNA sequences of these isolates confirmed they are
members of the genus Nosema. Based on the result of Western-blot hybridization with the rabbit anti-Nosema spodopterae spore
antiserum, three serotypes could be distinguished: N. spodopterae (S. litura isolate) and Pi. spp. isolate; S. exigua and H. armigera
isolates; and P. xylostella isolate. The amplicons of RAPD-PCR with 60 primers yielded clear patterns that were selected and used
for identification and also for phylogenic analysis of these five isolates. Based on analysis by the computer, isolates could be clearly
divided into three groups that were coincident with the serotypes; therefore we suggest that N. spodopterae and isolates of Pi. spp., S.
exigua, and H. armigera are more closely related in phylogenesis. In addition, in the amplification with the Nosema bombycis specific
primer set, only DNAs from P. xylostella isolate and N. bombycis yielded amplicons. Therefore, we suggest that four isolates,
excludingthe P. xylostella isolate, are N. spodopterae, and the taxonomic position of P. xylostella isolate needs to be elucidated.
2003 Elsevier Science (USA). All rights reserved.
Subjects
Nosema spp.
Infectivity
RAPD
SSUrDNA
Western-blot hybridization
flue gas desulfurization
Ca(OH)(2)
fly ash
structural properties
pollution control
Publisher
Taipei:National Taiwan University Dept Chem Engn
Type
journal article
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