Characterization of the Resistance to Callosobruchus maculatus (F.) in a Mungbean Variety VC6089A and Its Resistance-Associated Protein VrD1
Date Issued
2004
Date
2004
Author(s)
Lin, Chan
DOI
en-US
Abstract
Characteristics of resistance of VC6089A, a mungbean (Vigna radiata) variety bred by utilizing a wild Vigna species, Vigna sublobata (Accession TC1966), and containing a novel protein VrD1, was investigated against cowpea weevil, Callosobruchus maculatus (F.). Seeds of VC6089A showed high level of resistance; more than 96% of the bruchid eggs failed to develop into adults, whereas over 80% of eggs laid on susceptible cultivar VC1973A became adults. Mortality of surviving bruchids raised for five generations on VC6089A remained higher than 96%; however, female adults maintained high fecundity through these generations. This positive growth of bruchid population reared on seeds of VC6089A may result in bruchid population eventually developing resistance to the resistant mungbean. The protein VrD1 purified from seeds of VC6089A showed marked toxicity to C. maculatus when insects were reared on artificial seeds containing varying levels of VrD1. Thorough inhibition of development was observed when artificial seeds containing merely 0.2% (w/w) VrD1 was used for insect feeding. VrD1 appears to be one of the most potent anti-bruchid proteins discovered from plant sources so far. But it seems not to be the principle factor responsible for bruchid resistance in VC6089A seed, considering the discrepancy between the toxicity of VrD1 and the resistant seed. The mechanism of VrD1 toxicity to C. maculatus needs to be investigated further. Our findings demonstrated the insecticidal activity of VC6089A seeds and VrD1 protein against C. maculatus. These results may facilitate safer control against bruchid infestation, but the potential of these pests developing resistance to the resistant mungbean variety through maintaining high fecundity should be carefully monitored and managed as well.
Subjects
VC6089A
四紋豆象
抗蟲蛋白
VrD1
Bruchid resistance
Callosobruchus maculatus
Type
thesis
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