The association of membrane frizzled-related protein (MFRP) gene with acute angle-closure glaucoma - A pilot study
Journal
Molecular Vision
Journal Volume
14
Pages
1673-1679
Date Issued
2008
Author(s)
Abstract
Purpose: The membrane frizzled-related protein (MFRP) has been proposed as a probable candidate gene for extreme hyperopia and nanophthalmos, which are factors for angle-closure glaucoma. The purpose of our study was to investigate whether there are significant associations between angle-closure glaucoma and sequence variants in the MFRP gene reported previously in Taiwanese subjects. Methods: Genomic DNA was collected from 63 subjects with angle-closure glaucoma and 66 age-matched and gender-matched controls without angle-closure glaucoma. Three sequence variants were detected by polymerase chain reaction (PCR) and direct sequencing in all of the cases and controls. Results: None of the three sequence variants showed a significant result in terms of association with disease. The pairwise linkage disequilibrium (LD) mapping confirmed that these alleles have a comparatively strong LD index greater than 0.7 for D' and greater than 0.4 for r2 at these polymorphisms. However, we found there were no statistical associations between any of the three sequence variants located on MFRP and angle-closure glaucoma. Conclusions: In our pilot study, variations that we tested in MFRP were not associated with the development of acute angle-closure glaucoma in Taiwanese subjects. ? 2008 Molecular Vision.
SDGs
Other Subjects
frizzled protein; genomic DNA; membrane frizzled related protein; membrane protein; methionine; unclassified drug; valine; adult; amino acid substitution; article; closed angle glaucoma; controlled study; disease association; female; gene frequency; gene linkage disequilibrium; hospital based case control study; human; major clinical study; male; pilot study; polymerase chain reaction; priority journal; sequence analysis; single nucleotide polymorphism; Taiwan; Case-Control Studies; Female; Genetic Predisposition to Disease; Glaucoma, Angle-Closure; Haplotypes; Humans; Linkage Disequilibrium; Male; Membrane Proteins; Middle Aged; Pilot Projects; Polymorphism, Single Nucleotide; Sequence Analysis, DNA
Type
journal article