Retention Behavior of Highly Hydrophobic, Structurally Related Corticosteroids with SDS Based Microemulsion
Resource
Journal of Liquid Chromatography & Related Technologies 32 (6): 833-848
Journal
Journal of Liquid Chromatography & Related Technologies
Pages
833-848
Date Issued
2009
Date
2009
Author(s)
Wu, Chi-Hung
Chen, Tse-Hsien
Wang, Guan-Ren
Huang, Kuan-Pin
Liu, Chuen-Ying
Abstract
Abstract The present work describes the retention behavior of structurally related corticosteroids separated with SDS based microemulsion. The analytes include cortisone acetate (CA), hydrocortisone (H), hydrocortisone acetate (HA), prednisolone (P), prednisolone acetate (PA), and prednisone (Ps), which are not easily separated by the conventional MEKC. Surfactants with linear and planar structures have been compared for the separation of these analytes. By fine tuning the parameters, the results revealed that complete baseline separation could be achieved with a microemulsion consisted of 0.8% (w/w) n-octane, 3.6% (w/w) SDS, 6.6% (w/w) 1-butanol, and 89% (w/w) phosphate buffer (40 mM, pH 8.0), under an applied voltage of +7 kV and a column dimension of 53 cm (effective length, 30 cm) × 75 μm id. Although the composition of the microemulsion has a high recognition property for these structurally related compounds, the high affinity of these analytes with the pseudostationary phase made the analysis time very long. The effects of the addition of acetonitrile, methanol, and isopropanol have also been studied. Acetonitrile was found to have the greatest impact on this separation.
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Type
journal article
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