Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein
Resource
Biochemical and Biophysical Research Communications 372 (1): 67-72
Journal
Biochemical and Biophysical Research Communications
Pages
67-72
Date Issued
2008
Date
2008
Author(s)
Noisakran, Sansanee
Sengsai, Suchada
Thongboonkerd, Visith
Kanlaya, Rattiyaporn
Sinchaikul, Supachok
Chen, Shui-Tein
Puttikhunt, Chunya
Kasinrerk, Watchara
Limjindaporn, Thawornchai
Wongwiwat, Wiyada
Malasit, Prida
Yenchitsomanus, Pa-thai
Abstract
Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. ? 2008 Elsevier Inc. All rights reserved.
Subjects
Dengue virus; hnRNP C1/C2; Host and viral protein interaction; Interacting protein; NS1; Virus replication
SDGs
Other Subjects
heterogeneous nuclear ribonucleoprotein C; heterogeneous nuclear ribonucleoprotein c1; heterogeneous nuclear ribonucleoprotein c2; nonstructural protein 1; virus protein; article; cell lysate; cell strain HEK293; cell survival; controlled study; dengue; Dengue virus; embryo; embryo cell; host cell; human; human cell; immunoprecipitation; kidney cell; mass spectrometry; nonhuman; priority journal; protein analysis; protein localization; tandem mass spectrometry; time of flight mass spectrometry; two dimensional gel electrophoresis; virus cell interaction; virus pathogenesis; virus replication; Antibodies, Monoclonal; Cell Line; Cell Nucleus; Cytoplasm; Dengue Virus; Heterogeneous-Nuclear Ribonucleoprotein Group C; Humans; Immunoprecipitation; Protein Isoforms; Proteomics; Tandem Mass Spectrometry; Viral Nonstructural Proteins; Virus Replication; Dengue virus
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