Preparation and characterization of RGD-immobilized chitosan scaffolds
Resource
Biomaterials 26 (16): 3197-3206
Journal
Biomaterials
Journal Volume
26
Journal Issue
16
Pages
3197-3206
Date Issued
2005
Date
2005
Author(s)
Ho, Ming-Hua
Liu, Hwa-Chang
Hsien, Tzu-Yang
Lai, Juin-Yih
Hou, Lein-Tuan
Abstract
Chitosan scaffolds were modified with RGDS (Arg-Gly-Asp-Ser) in the present work via an imide-bond forming reaction between amino groups in chitosan and carboxyl groups in peptides. Successful immobilization was verified with FTIR spectroscopy, and the immobilized amount was determined to be on the order of 10 -12 mol/cm 2 through analysis of the immobilized amino acids. Results of experiments of cell culture with rat osteosarcoma (ROS) cells demonstrated that RGDS immobilization could enhance the attachment of ROS cells onto the chitosan, resulting in higher cell density attached to the RGDS-modified scaffold than to the unmodified scaffold. It should be noted that only RGDS, but not other peptide such as RGES, is effective in enhancing cell attachment and possible proliferation. Experiments of in vitro mineralization indicated that there were more cells on the RGDS-modified scaffold than on the unmodified scaffold, which tended to form bone-like tissues. The results presented in this work suggest that immobilization of RGDS can make chitosan scaffolds more compatible for the culture of osteoblast-like cells and the regeneration of bone-like tissues. © 2004 Elsevier Ltd. All rights reserved.
Type
journal article
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