Studies on the Leaf Characters and ISSR DNA Variation of Oiltea Camellia Germplasm in Taiwan
Date Issued
2010
Date
2010
Author(s)
Lin, Shang-Yi
Abstract
Genetic variation of leaf characters and ISSR DNA markers for 33 Camellia oleifera accessions (collected from 3 areas) and 46 Camellia tenuifolia accessions (collected from 5 different areas) in Taiwan was studied. Based on seven leaf traits, including leaf length, leaf width, leaf thickness, leaf ratio, numbers of serrations, percentage of difference between actual length of leaf and the natural ones and percentage of difference between actual width of leaf and the natural ones, principle component analysis result could divide C. oleifera and C. tenuifolia apart clearly. Formula established by multivariate discriminate analysis is very powerful and reliable to distinguish the oiltea Camellia species. Coefficients of variation (CV) of leaf length, leaf width, leaf thickness, leaf ratio and numbers of serrations are around 10 %, suggesting the satisfactory stability of the traits. These traits could be catalogued and used for variety identification.
Molecular level variation was revealed by 11 ISSR primers which could stably generate 31 robust polymorphic bands. Similar results were found in cluster analysis and principle component analysis based on ISSR markers. The ISSR DNA marker similarities within species are 54.8 % in C. tenuifolia and 58.9 % in C. oleifera. The result of AMOVA shows that the variation component within species is larger than between species and both regional variation components of C. oleifera and C. tenuifolia are statistically significant (26.42 % and 37.41 %). The variation within regions is larger than among regions indicating that high genetic variation is existed in C. oleifera and C. tenuifolia populations. POPGENE analysis result shows both C. oleifera and C. tenuifolia have the higher Nei''s coefficients of gene variation (Gst) values than common insect pollination plants. The estimates of gene flow from both Gst values were below 1, implying the existence of differentiation among different populations of a species.
Subjects
C. oleifera
C. tenuifolia
leaf characters
genetic variation
Type
thesis
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