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  4. Integrated centrifugal microfluidic device with three-dimensional molecular concentrator for plasma separation and biomarker enrichment.
 
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Integrated centrifugal microfluidic device with three-dimensional molecular concentrator for plasma separation and biomarker enrichment.

Journal
Journal of chromatography. A
Journal Volume
1757
Start Page
Article number 466146
ISSN
1873-3778
Date Issued
2025-06-12
Author(s)
Xu, Wei-Chen
Lu, Jun-Yan
CHUNG-YU CHEN  
Kuo, Ju-Nan
DOI
10.1016/j.chroma.2025.466146
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/730631
Abstract
Microfluidic-based liquid biopsy allows for sensitive and non-invasive detection of circulating biomarkers such as circulating free DNA (cfDNA), circulating tumor cells (CTCs), and extracellular vesicles (EVs), serving as a valuable tool for early cancer diagnosis, monitoring, and precision medicine. The clinical application of the prostate-specific antigen (PSA) has significantly improved prostate cancer screening. The integration of microfluidics with PSA detection enables ultra-sensitive analysis at low concentrations, crucial for early diagnosis, prompt treatment, and reduced metastasis and mortality. In this research, a novel centrifugal microfluidic device combined with a three-dimensional molecular concentrator was created to separate and quantitatively decant blood plasma from whole blood samples and concentrate PSA biomarker molecules. The volume of plasma entering the molecular concentrator was precisely regulated by adjusting the rotational speed. The electrokinetic behavior of the molecular concentrator was studied through numerical modeling and experimental validation. The incorporation of a microconstriction structure significantly improved electric field localization, enhancing the field-focusing factor by approximately 30,000-fold. This intensified field facilitated rapid molecular accumulation, resulting in a 10-fold increase in PSA within 55 s. This device allows for quick and highly sensitive biomarker detection from minimally processed blood, providing a scalable and cost-effective platform for early cancer diagnosis and precision medicine.
Subjects
Biomarker
Centrifugal microfluidic device
Molecular concentrator
PSA
Plasma
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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