Anti-diabetic effect of a traditional Chinese medicine formula
Journal
Food and Function
Journal Volume
3
Journal Issue
11
Pages
1161-1169
Date Issued
2012
Author(s)
Wang H.-J.
Abstract
An anti-diabetic TCM formula consisting of Schizandra chinensis Baill. (SC), Coptis chinensis (CC), Psidium guajava L. leaves (PG) and Morus alba L. leaves (MA) was developed based on its £\-glucosidase, DPP-4 and AGE inhibitory activities in vitro using response surface methodology (RSM). Then, the in vivo study was carried out to confirm the anti-diabetic function of the formula. RSM results showed that the optimum anti-diabetic TCM formula is the combination SC (3000 £gg mL-1), CC (80 £gg mL-1), PG (374.56 £gg mL-1) and MA (480 £gg mL-1). For the in vivo study, insulin resistant mice were induced by high-fat/high-sucrose (HF/HS) feeding for 6 weeks. Administration of the developed formula significantly decreased non-fasting blood glucose in the HF/HS diet mice. Moreover, the formula decreased blood glucose levels in the insulin tolerance test. These results indicated that the anti-diabetic mechanism of the formula might be due to decreased insulin resistance. The serum fructosamine level in the high dose group was significantly lower than the HF/HS and normal control groups, indicating that the formula could improve middle term glucose levels and reduce the risk of complications. The contents of berberine and 1-deoxynojirimycin in the formula were 4.7 ¡Ó 0.4 and 77.1 ¡Ó 1.1 £gg mL-1, respectively. These two compounds can be used as indicators for quality control during production. ? The Royal Society of Chemistry.
SDGs
Other Subjects
Blood; Glucose; Mammals; Sugar (sucrose); Blood glucose; Blood glucose level; Glucose level; Glucosidase; High dose; In-vitro; In-vivo; Inhibitory activity; Insulin resistance; Morus alba; Normal controls; Psidium guajava; Response surface methodology; Traditional Chinese Medicine; Insulin; Coptis chinensis; Morus alba; Mus; Psidium; Psidium guajava; Schisandra chinensis; Schisandraceae; advanced glycation end product; alpha glucosidase; antidiabetic agent; diagnostic agent; dipeptidyl peptidase IV; dipeptidyl peptidase IV inhibitor; DPP4 protein, human; herbaceous agent; insulin; animal; article; C57BL mouse; chemistry; Coptis; drug antagonism; experimental diabetes mellitus; glucose tolerance test; insulin resistance; male; Morus; mouse; phytotherapy; plant leaf; Psidium; Schisandra; alpha-Glucosidases; Animals; Coptis; Diabetes Mellitus, Experimental; Dipeptidyl Peptidase 4; Dipeptidyl-Peptidase IV Inhibitors; Drugs, Chinese Herbal; Glucose Tolerance Test; Glycosylation End Products, Advanced; Hypoglycemic Agents; Insulin; Insulin Resistance; Male; Mice; Mice, Inbred C57BL; Morus; Phytotherapy; Plant Leaves; Psidium; Schisandra
Type
journal article