Study of classifying sperms with different motility in a microfluidic platform
Date Issued
2009
Date
2009
Author(s)
Huang, Zi-Wei
Abstract
Assisted reproductive technology (ART) like intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) reflect the importance of functional spermatozoa pre-selecting. Motility-based sperm preservation to provide competent sperm for clinic use is required. In addition, sperm quality examination such as sperm concentration, motility, and morphology are the index of the male infertility. The uniform standard for diagnosing male infertility is another significant issue. This thesis presents of sperm motion analysis to classify sperm motility via a microfluidic device in order to achieve assisted concepts. Based on the self- movement and against-flow phenomenon of motile sperm, the device can also isolate motile sperm from any other cellular debris. The device, an inexpensive microchip, can integrate observation, isolation, and classification of motile sperm in one chip without the need for electronics or external syringe pump. Hydrostatic pressure was used as the steady driving source in the three reservoirs: semen sample inlet, buffer inlet, and motile sperm outlet, where the flow field can be controlled specifically in three groups (maximum velocities are 10μm/s, 30μm/s, and 50μm/s), and motile sperm will be selected by the motility of each individual sperm.esults show successful separation via sperm motility. Comparatively, the motile sperm ratio was high (near 100%) after sorting, and the motility-based sperm classification show the positive relationship during the experiment. The uniform standard of sperm classification make it possible to diagnose the state of infertility, or even the health of the individual, it reduce the artificial mistake of determining sperm motility in whole semen sample and save a quantity of time and labor from diagnosis. The device provided a small, portable, and disposable platform on which to deal with issues, such as classification based sperm bank, ICSI, IVF etc, and should be useful to address clinical need of uniform standard for sperm classification.
Subjects
microfluidic
sperm
motility
classify
spermatozoa
microfluidics
Type
thesis
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