Producing hydrogen from phenol-containing wastewater using Clostridum butyricum
Date Issued
2009
Date
2009
Author(s)
Tai, Jung
Abstract
This thesis focus on the research of producing hydrogen in phenol-containing synthetic wastewater using Clostridium butyricum which has very good ability of hydrogen production by degrading hydrocarbons. And we use glucose and cellobiose as the main carbon source its concentration to culture and observe its resistance to phenol and ability of hydrogen production.uring batch experiment, we discover C.butyricum had bood performace of hydrogen production in toxic surrounding. The highest hydrogen production of C.butyricum is 1.46 mole hydrogen．mole-1 glucose and 2.11 mole hydrogen．mole-1 cellobiose when using glucose and cellobiose as main carbon source. We also found phenol was degraded during glucose and cellobiose fermentation.n cellobiose fermentation batch test, we found C.butyricum can produce higher hydrogen yield in phenol-containing medium than no phenol and the highest hydrogen yield is 2.11 at 600 ppm. The activity of laccase also was highest at 600 ppm. Maybe more phenol was cracked and converted to pyruvate by laccase and pyruvate was converted to acetyl-CoA and released hydrogen. Therefore more hydrogen was produced at 600 ppm phenol concentration. As the phenol concentration over 600 ppm the hydrogen yield began to decrease because high phenol concentration inhibit C.butyricum growth. Hydrogenase activity was decrease as phenol concentration increase. The activity of ydrogenase was highest when no phenol inside and cut down immediately in the presence of phenol. But it had a gap between overall hydrogen production rate and enzymatic hydrogen production rate. The difference maybe from membrane-bound hydrogenase. Membrane-bound hydrogenase activity was low gradually because the inhibition of phenol.
Subjects
Clostridium butyricum
hydrogen
wastewater
SDGs
Type
thesis
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