Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
6426
Date Issued
2007
Author(s)
Abstract
The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating the structural alterations and the pattern of microbial spreading during corneal infectious process in an in vitro organ culture model. The autofluorescence spectrum derived from pathogens allows us to monitoring the pattern of microbial spreading within corneal lamellae. In addition, the destruction and regeneration of second harmonic generating collagen during infectious process can also be monitored in a non-invasive fashion. Therefore we propose that multiphoton microscopy may potentially be applied as an effective monitoring tool for corneal infection studies.
Type
conference paper
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