Host preference and trait variations of Liriomyza trifolii (Diptera: Agromyzidae)
Date Issued
2006
Date
2006
Author(s)
Ko, Nai-Wen
DOI
zh-TW
Abstract
Liriomyza trifolii is a highly polyphagous and cosmopolitan pest. According to the investigation, the host range of L. trifolii has extensive embraced more than 400 species in 25 families. Females of L. trifolii lay eggs in the leaves; after hatching, larvae feed in the leaves and will not leave until pupation. Actually, females lay eggs in which host will determine the fate of the larvae; and that indicates the importance of the host preference of females. In this study, I used kidney bean (Phaseolus vulgaris) to rear L. trifolii stock at 24.5 ± 1˚C, 70 ± 10% R.H., photoperiod 12L : 12D and offered beggar-tick (Bidens alba), kidney bean, rape (Brassica campestris), and tomato (Lycopersicon escultentum) as foods for females to test their host preference. The results showed that females exhibited significantly feeding and ovipositional preference on beggar-tick, and larvae ate in kidney bean have highest pupal weight, survivorship and emergence rates. On the other hand, another colony reared on rape was also established. After 13 generations, the host preferences of the two colonies were compared. The data showed that kidney bean colony has significantly feeding and ovipositional preference for kidney bean, but rape colony has no significant difference for the preference between the two plants. Moreover, I also analysed the traits variation for these two populations. For morphometric studies, 17 landmarks data from connective points of wing veins were selected and used for superimposition and Thin-plate spline-based analysis to detect the morphological variations among populations. The results showed there have directional variations on the endpoints of inner and outer cross veins in each generation. As time goes by, it seems that there are two different, potential directions of the sample variations of the two colonies. Phylogenetic relationships among populations were also reconstructed by using DNA sequencing data of about 1,018 bp of mitochondrial cytochrome oxidase subunit I and II genes. Unfortunately, I did not find any useful molecular markers that can successfully divide those two colonies from different host plants. Meanwhile, the data of total protein analysis can not show the differences between colonies neither.
Subjects
非洲菊斑潛蠅
寄主偏好
幾何形態測量
特徵變異
Liriomyza trifolii
host preference
geometric morphometrics
trait variations
Type
thesis
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