Selection of rhizobacteria from Lilium formosanum and the application for the control of Botrytis leaf blight
Date Issued
2004
Date
2004
Author(s)
Liu, Yi-Hung
DOI
zh-TW
Abstract
Stimulations of biotic or abiotic factors alter physiological or biochemical reactions of plants to evolve multiple complex defense mechanisms for resistance to adverse stress and pathogen infection. So far, plant defense mechanisms include pre-existed and induced resistance. Induced resistance of plants includes systemic acquired resistance (SAR)、induced systemic resistance (ISR) and hypersensitive response (HR). Botrytis leaf and blossom blight caused by Botrytis elliptica (Berk.) Cooke is an important disease for the bulb and flower productions of Lilium spp. and causes severe losses of yield and value of lilies in Taiwan. For biocontrol of Lilium formosanum Wall., application of rhizobacteria was proposed for practical use. Sixty-two bacterial strains were isolated and purified from L. formosanum rhizosphere soil by dilution plate method and identified according to the fatty acid profiles by MIDI identification system. Bacterial strains promoting the growth of the seedling of Formosa lily, without negative effect, were selected. The greenhouse and field disease control assay showed that the disease severity of Botrytis leaf blight could be decreased effectively by application of bacterial suspension of Bacillus spp. and Pseudomonas spp. in the rhizosphere of lily plants. For cloning of defense-related genes or molecular markers for the induced resistance of L. formosanum, PCR-select cDNA subtraction was implemented to screen out the rhizobacterium-responsive genes. Among 193 clones, twenty-three were differentially expressed as indicated by dot blot hybridization.
Subjects
生物防治
百合灰黴病菌
根圈細菌
誘導系統性抗病
biocontrol
Botrytis elliptica
Formosa lily
Rhizobacteria
induced systemic resistance (ISR)
Type
other
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