Motorcycle exhaust particles up-regulate expression of vascular adhesion molecule-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells
Journal
Toxicology in Vitro
Journal Volume
26
Journal Issue
4
Pages
552-560
Date Issued
2012
Author(s)
JAW-JOU KANG
Abstract
Epidemiological studies have shown that there is a strong correlation between atherosclerosis and ambient air pollution. In this study, we found that motorcycle exhaust particles (MEP) induced adhesion between cells of the human monocytic leukemia cell line (THP-1) and human umbilical vein endothelial cells (HUVECs) in a time-and dose-dependent manner. In addition, MEP treatment induced both mRNA and protein expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in HUVECs. The IκB degradation and p65 nuclear translocation was found in MEP-treated HUVECs, suggested the involvement of nuclear factor-κB (NF-κB). MEP-induced VCAM-1 and ICAM-1 protein expression was inhibited by NF-κB inhibitor BAY 11-7085. Oxidative stress was also involved in the signaling of VCAM-1 and ICAM-1 expression. MEP treatment caused hydrogen peroxide and superoxide formation. Pretreatment with α-tocopherol could inhibit MEP-induced reactive oxygen intermediates generation and suppressed MEP-induced IκB degradation and adhesion molecules expression. Furthermore, the carbon black (CB) nanoparticles with different diameters could induce VCAM-1 and ICAM-1 protein expression; however, polycyclic aromatic hydrocarbons (PAHs) only increased the expression of ICAM-1 but not that of VCAM-1 in HUVECs. In this study, we found that MEPs could induce ICAM-1 and VCAM-1 expression through oxidative stress and NF-κB activation in HUVECs. ? 2012 Elsevier Ltd.
Subjects
Cell adhesion molecule; Motorcycle exhaust particle; NF-κB; Oxidative stress; Polycyclic aromatic hydrocarbons
SDGs
Other Subjects
3 (4 tert butylphenylsulfonyl) 2 propenenitrile; alpha tocopherol; cell adhesion molecule; chrysene; fluoranthene; hydrogen peroxide; I kappa B beta; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; phenanthrene; polycyclic aromatic hydrocarbon; pyrene; superoxide dismutase; synaptotagmin I; tumor necrosis factor alpha; vascular adhesion protein 1; article; cancer cell culture; cell adhesion; controlled study; dose response; endothelium cell; exhaust gas; human; human cell; immunoblotting; motorcycle; oxidative stress; protein degradation; protein expression; reverse transcription polymerase chain reaction; signal transduction; umbilical vein; upregulation; Western blotting; Air Pollutants; Cell Adhesion; Cell Line, Tumor; Human Umbilical Vein Endothelial Cells; Humans; Intercellular Adhesion Molecule-1; Motorcycles; NF-kappa B; Oxidative Stress; Polycyclic Hydrocarbons, Aromatic; RNA, Messenger; Soot; Up-Regulation; Vascular Cell Adhesion Molecule-1; Vehicle Emissions
Type
journal article