Application of Metal Nanoparticles (MNPs) Labeling to DNA Microarray
Date Issued
2009
Date
2009
Author(s)
Wu, Cheng-Chu
Abstract
DNA microarray is a powerful tool allowing simultaneous detection of many different target molecules present in a sample. By using these techniques, large-scale parallel analyses can be performed in short time periods. Fluorescence technology has been the gold standard for detection of DNA on microarrays due to its high sensitivity, dynamic range, and multiplexing capabilities. Despite these attributes, fluorescence labeling typically requires target amplification to obtain sufficient amounts of target and complex instrumentation is needed for detection, ultimately limiting its utility in the applications described above. he development of a metal nanoparticle-based detection methodology for sensitive and specific DNA microarray is described. The technology utilizes gold and silver nanoparticles derivatives with thiol modified oligonucleotides that are designed to bind complementary DNA targets. Scattered light from nanoparticle-tagged biomolecules can be imaged and quantified extremely sensitively. It allows for detection and quantization by measuring evanescent wave induced light scatter with low-cost optical detection systems. Compared to Cy3-based fluorescence, gold nanoparticle probes provide for a 1000-fold increase in sensitivity.
Subjects
microarray
gold nanoparticle
scattering spectra
Type
thesis
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