行政院國家科學委員會補助專題研究計畫 成果報告:台灣地區早發性乳癌之分子細胞學研究 ─台灣地區早發性乳癌在細胞凋亡調控基因之研究
Date Issued
2003
Date
2003
Author(s)
DOI
912320B002161
Abstract
Breast cancers are one of the most important
and common tumors in humans. The etiology of
breast cancers is complex. The secreted frizzled
related proteins (sFRPs) are newly identified
proteins and implicated to have dual roles of
modulation of Wnt-Frizzled signal transduction
pathway and regulation of apoptosis. We have
recently found that sFRP2 was expressed
abundantly in human breast cancer (mostly late
onset) and canine mammary gland tumors (MGT)
tissues but was undetectable in normal mammary
glands. To systematically investigate the functional
roles and molecular mechanisms of sFRP2 in early
onset breast cancers, several strategies are to be
carried out as described below.
The project was intended to be comprised of six
major parts for a period of 3 years. However, only
one year of funding was granted. During this year,
breast cancer tissues and primary cell cultures from
native early onset breast cancer tissues have been
established and purified for epithelial cells. We
have successfully analyzed the sFRP2 expression in
early onset breast cancer tissues. In addition, we
have established and analyzed native primary early
onset breast cancer cell lines from surgically
excised breast cancer specimens. The cells are
characterized for their cell origins, expression of
sFRP2 by RT-PCR, in situ hybridization, and
immunohistochemistry. Expression experiments
revealed the sFRP2 was abundantly expressed with
significantly a higher expression rate in early onset
breast cancer tissues (86% mRNA positive), while
much lower expression rate in normal breast
tissues (43% mRNA positive). In the following
step, human sFRP2 is cloned into a mammalian
expression vector with GFP reporter gene and
CMV promoter. The GFP-sFRP2 is stably
transfected into primary early onset breast cancer
cell lines by lipofection for further analysis from
the next stage of the project although no funding is
granted for the next year’s study.
The results of this stage of the project
should offer important scientific basis and
information to understand the roles of sFRP gene
family in early onset breast cancers. It also
provides a basis for further analysis of functions of
different members of the sFRP gene family and
elucidation of the complex etiology of early onset
breast cancers.
Subjects
secreted apoptosis related protein
secreted frizzled related protein
apoptosis
gene
expression
expression
gene transfection
early onset breast
cancer
cancer
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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