Biomaterial Substrate-Mediated Multicellular Spheroid Formation and Their Applications in Tissue Engineering
Journal
Biotechnology Journal
Journal Volume
12
Journal Issue
12
Date Issued
2017
Author(s)
Abstract
Three-dimentional (3D) multicellular aggregates (spheroids), compared to the traditional 2D monolayer cultured cells, are physiologically more similar to the cells in vivo. So far there are various techniques to generate 3D spheroids. Spheroids obtained from different methods have already been applied to regenerative medicine or cancer research. Among the cell spheroids created by different methods, the substrate-derived spheroids and their forming mechanism are unique. This review focuses on the formation of biomaterial substrate-mediated multicellular spheroids and their applications in tissue engineering and tumor models. First, the authors will describe the special chitosan substrate-derived mesenchymal stem cell (MSC) spheroids and their greater regenerative capacities in various tissues. Second, the authors will describe tumor spheroids derived on chitosan and hyaluronan substrates, which serve as a simple in vitro platform to study 3D tumor models or to perform cancer drug screening. Finally, the authors will mention the self-assembly process for substrate-derived multiple cell spheroids (co-spheroids), which may recapitulate the heterotypic cell–cell interaction for co-cultured cells or crosstalk between different types of cells. These unique multicellular mono-spheroids or co-spheroids represent a category of 3D cell culture with advantages of biomimetic cell–cell interaction, better functionalities, and imaging possibilities. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Subjects
cancer cells; chitosan; mesenchymal stem cells; spheroids; tissue engineering
SDGs
Other Subjects
Biomimetics; Body fluids; Cell culture; Cells; Chitin; Chitosan; Cytology; Diagnosis; Diseases; Self assembly; Stem cells; Substrates; Tissue; Tissue engineering; Tumors; Cancer cells; Cell-cell interaction; Mesenchymal stem cell; Multicellular aggregates; Multicellular spheroid; Regenerative medicine; Self assembly process; Spheroids; Cell engineering; biomaterial; chitosan; cytokine; growth factor; hyaluronic acid; angiogenesis; cancer research; cartilage; cell aggregation; cell differentiation; cell interaction; cell proliferation; cell regeneration; chemical structure; drug screening; human; mesenchymal stem cell; monolayer culture; multicellular spheroid; nerve regeneration; nonhuman; priority journal; Review; spheroid cell; tissue engineering; tissue regeneration; tumor model; tumor spheroid; animal; cell line; cytology; medical research; mesenchymal stroma cell; mouse; physiology; Animals; Biocompatible Materials; Biomedical Research; Cell Line; Humans; Mesenchymal Stromal Cells; Mice; Spheroids, Cellular; Tissue Engineering
Type
journal article