De-emulsification of oil-in-water emulsion by Rhodococcus erythropolis NTU-1 bioflocculi grown in diesel
Date Issued
2014
Date
2014
Author(s)
Lai, Yueh-Ting
Abstract
NTU-1, a highly efficient demulsifying strain, was isolated from petroleum contaminated soil and identified as Rhodococcus erythropolis by 16S rDNA gene analysis. NTU-1 powder with high demulsifying performance could be obtained by heat-drying after collecting bioflocculi in medium.
This study first evaluated the demulsifying performance of NTU-1 cultured by diesel and n-hexadecane, and found that NTU-1 powder by diesel is a better choice for its lower cost and better performance. At an optimal quantity of dried NTU-1 powder for de-emulsification that was proportional to the volume of Tween-80 to the 2/3 power, a recovery of 95 % oil was achieved in 480 min. This was made possible when the oil/water/Tween80 volume ratio was set at 4/1/0.01. The higher O/W ratio and the lower viscosity both contributed to such a high oil recovery rate.
The positive correlation between CSH (MATH) and oil recovery ratio also implies that the diesel grown NTU-1 culture may provide a commercial scale-up method to break oil -in-water emulsion directly at the primary oil recovery site.
Subjects
Rhodococcus erythropolis NTU-1
破乳化
油相回收百分比
細胞表面疏水性
水包油(O/W)型乳化液
Type
thesis
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