Purification of Epstein-Barr virus associated DNase with affinity chromatography of nasopharyngeal carcinoma patient serum.
Journal
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology
Journal Volume
23
Journal Issue
1
Start Page
34
End Page
43
ISSN
0253-2662
Date Issued
1990-02
Author(s)
Abstract
Epstein-Barr virus associated DNase in the homogeneous form can be purified by chromatographys using CH-sepharose 4B column conjugated with nasopharyngeal carcinoma patient serum, DNA cellulose and phosphocellulose in that sequence. The molecular weight of this enzyme is shown to be 51 kilo-daltons in silver-staining and immunostains. Among various methods for keeping DNase activity, the addition of BSA and dialysis in glycerol or ethylene glycol immediately after the enzyme purification is suggested.
Type
journal article