Various Microscopic Methods for Investigating the Venuloid Idioblasts of Pteris grevilleana Wall
Resource
Taiwania, 53(4), 394-400
Journal
Taiwania
Journal Volume
53
Journal Issue
4
Pages
394-400
Date Issued
2008-12
Date
2008-12
Abstract
Venuloid idioblasts are vein-like structures. In Pteris, they are long epidermal cells with very thick cell walls. In this study, venuloid idioblasts of Pteris grevilleana were investigated with various light microscopic (LM) and scanning electron microscopic (SEM) techniques and the main purposes of these microscopic techniques are summarized and discussed. To investigate the morphology and distribution of venuloid idioblasts, partial polarization LM technique and cryo-tabletop-SEM technique were used. The idioblasts had lobed margins, acute or round ends, and could be found on both upper and lower epidermis of fertile fronds and sterile fronds. They are distributed on veins, interveinal regions, and leaf margins, but not on costules, costae, and false indusia. By using histochemical staining and SEM with energy dispersive X-ray spectrometer (EDS), it was confirmed that the idioblasts contained silicon. In addition, the venuloid-idioblast-like silica bodies were extracted by wet oxidation method. These indicate that the venuloid indioblast in P. grevilleana is a kind of spicular cell (long epidermal cells containing silica bodies, which are found in Adiantoids and Vittarioids).
Subjects
EDS
false vein
partial polarization
Pteridaceae
Pteris grevilleana
silica body
spicular cell
tabletop SEM
venuloid idioblast
wet oxidation
能量分散X射線光譜儀
假脈
半偏光
鳳尾蕨科
翅柄鳳尾蕨
矽酸體
矽異形細胞
桌上型掃瞄式電子顯微鏡
Type
journal article
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