Investigation of Transcriptional Regulation of AtSUS1 and AtSUS4 in Arabidopsis during Flooding
Date Issued
2011
Date
2011
Author(s)
Su, Wei-Chu
Abstract
Oxygen diffusion is about 10 000 times slower in water than that in air. When plants were subjected to flooding the root cells switch from aerobic respiration to anaerobic fermentation in response to oxygen deprivation (hypoxia). Cellular regulation was performed to increase the transcript levels of genes that are involved in mobilization of sugars, glycolysis and fermentation pathway. During submergence the expression of sucrose synthase (SUS) gene was up-regulated to catalyze degradation of sucrose into fructose and UDP-glucose, which are substrates for glycolysis. Although a large number of studies have been made on sucrose synthase gene function, little is known about the mechanism of transcriptional regulation of SUS genes under flooding. Here, we characterize gene expression patterns of the SUS gene family in Arabidopsis during flooding. The transcript levels of SUS1 and SUS4 increased in roots but in different manners during flooding. Expression cassettes with truncated promoter fragments of SUS1 and SUS4 linked to firefly (Photinus pyralis) luciferase coding sequence were also developed to detect promoter activity. The results from the 5’ promoter deletion analyses suggested that the region between -1000 and -600 (from transcription start site) in promoter of SUS4 was essential for flooding-induced gene expression. For SUS1 gene, the critical cis-element(s) may locate on -600~-300 region for transcriptional regulation under flooding.
Subjects
hypoxia
Arabidopsis
transcriptional regulation
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98b42012-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):f7700f6687211b4aa7692c352450ad17