Sequential cocultivation strategy for producing nisin-enriched foaming bacterial cellulose with enhanced antibacterial and functional properties
Journal
Cellulose
Journal Volume
32
Journal Issue
13
Start Page
7765
End Page
7782
ISSN
0969-0239
1572-882X
Date Issued
2025-07-31
Author(s)
Susilo, Marco Hadisurya
Lin, Hui-Wen
Hsieh, Chen-Che
Huang, Yi-Cheng
Chou, Yu-Chieh
Santoso, Shella Permatasari
Lin, Shin-Ping
Abstract
Bacteria cellulose (BC) is a natural biopolymer synthesized by Komagataeibacter xylinus (K. xylinus). To further enhance its functional properties, a foaming bacterial cellulose (FBC) with improved porosity and reswelling capacity was previously developed. In this study, an antimicrobial functionality was introduced to FBC by co-culturing K. xylinus with Lactococcus lactis, a nisin-producing lactic acid bacterium. The result demonstrated that the nisin titer was increased to 1.43-fold (from 3631.60 IU/mL−1 to 5427.39 IU/mL−1) utilizing 1:1 co-cultural ratio. Subsequently, most of the nisin-associated genes were upregulated during co-culture fermentation, nisA with 5.54-fold as the highest increasement. Furthermore, the others material properties: Fourier-transform infrared spectroscopy and thermogravimetric analysis, as well as the antibacterial zones around the nisin/FBC composites against Staphylococcus aureus indicates the successful nisin incorporation into FBC. Lastly, the nisin/FBC maintained the superior reswelling rates and non-toxicity properties of FBC. With this integrated strategy, the potential approach was established for upscaling nisin production, introducing an antimicrobial substance into the FBC, and uncovering the other applications of FBC.
Subjects
Co-culture
Foaming bacterial cellulose
Komagataeibacter xylinus
Lactococcus lactis
Nisin
SDGs
Publisher
Springer Science and Business Media LLC
Type
journal article