Rhodostomin Inhibits Thrombin-Enhanced Adhesion of Ros 17/2.8 Cells through the Blockade of αvβ3 Integrin

Osteosarcoma is a very malignant bone tumor which has a high metastatic potential and usually lead to poor prognosis. The adhesion of tumor cells to the endothelium or extracellular matrix (ECM) is an essential step in the metastatic cascade. We investigated the effect of thrombin on the adhesion activity of the osteosarcoma cell line, ROS 17/2.8. Incubation with the low concentrations of thrombin ( 0.01–5U/ml, 5min to 24h) elevated the adhesion activity of ROS 17/2.8 to both human umbilical vein endothelial cells ( HUVEC) and extracellular matrix, with the peak effect at the concentration of 0.5U/ml for 30min at 37°C. The ROS 17/ 2.8 cells responded to thrombin by a peak effect of increased adhesion to HUVEC (5.5 folds vs. control) and fibronectin (4.8folds) after thrombin pretreatment (0.5U/ml, 30min, 37°C). Pretreatment with monoclonal antibodies against β3 integrins, including anti-αvβ3, 10E5 and 7E3, effectively antagonized the thrombin-enhanced cell adhesion activity, whereas anti-α3 β1 and anti-α5β1 did not antagonize the enhanced cell adhesion. Rhodostomin, an Arg- Gly-Asp (RGD)- containing snake venom peptide, and synthetic peptide RGDS also blocked the thrombin-enhanced ROS 17/2.8 cell adhesion. This study demonstrated that thrombin enhanced the cell adhesion of ROS 17/2.8 cells to HUVEC or ECM through an upregulation of β3 integrins, and rhodostomin was a strong inhibitor on thrombin-enhanced cell adhesion, either to HUVEC or fibronectin substratum.