Clinical performance of multiplex high-risk e6 mrna expression in comparison with hpv dna subtypes for the identification of women at risk of cervical cancer
Journal
Journal of Medical Virology
Journal Volume
87
Journal Issue
8
Pages
1404-1412
Date Issued
2015
Author(s)
Abstract
We compared multiplex E6 messenger ribonucleic acid (mRNA) tests using real-time quantitative reverse transcriptase polymerase chain reactions (PCR) with human papillomavirus (HPV) DNA subtypes using a MY11/GP6+ PCR-based reverse-blot assay to identify cervical intraepithelial neoplasias of grade 2 or worse (CIN2+). In total, 684 women were studied, of whom 377 (55%) were diagnosed with CIN2+ histologically. The specificity of HPV mRNA to predict histological CIN2+ was higher than that of HPV DNA (81.3% vs. 44.2%). The odds ratios (ORs) to predict histological CIN2+ in women with positive for type 16, 18, 31, and 45 E6 mRNA or by HPV DNA detection were 7.1 (95% confidence interval [CI] 3.9-13.1) and 2.5 (95%CI 1.9-3.5), respectively, compared to those with negative for E6 mRNA or HPV DNA. The OR to predict histological CIN2+ in women with a cytological grade
Subjects
Cervical intraepithelial neoplasias; E6 messenger ribonucleic acid; Human papillomavirus
SDGs
Other Subjects
genomic DNA; messenger RNA; virus DNA; messenger RNA; oncoprotein; virus DNA; adult; aged; Article; cancer grading; cancer risk; controlled study; cross-sectional study; cytology; female; genotype; histopathology; human; Human papillomavirus type 16; Human papillomavirus type 18; Human papillomavirus type 31; major clinical study; reverse transcription polymerase chain reaction; uterine cervix cancer; uterine cervix carcinoma in situ; very elderly; Wart virus; Cervical Intraepithelial Neoplasia; comparative study; complication; evaluation study; genetics; mass screening; middle aged; molecular diagnosis; papillomavirus infection; predictive value; procedures; risk assessment; sensitivity and specificity; virology; young adult; Human papillomavirus; Adult; Aged; Aged, 80 and over; Cervical Intraepithelial Neoplasia; Cross-Sectional Studies; DNA, Viral; Female; Humans; Mass Screening; Middle Aged; Molecular Diagnostic Techniques; Oncogene Proteins, Viral; Papillomavirus Infections; Predictive Value of Tests; Risk Assessment; RNA, Messenger; Sensitivity and Specificity; Young Adult
Publisher
John Wiley and Sons Inc.
Type
journal article