石斑魚神經壞死病毒DNA疫苗之開發
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
陳秀男
DOI
932313B002057
Abstract
Nervous Necrosis Virus (NNV) has been demonstrated to be main causes for the cultured
marine fish worldwide. In recent decade, Taiwan, cultured groupers have been found to be
infected by NNV with a massive mortality especially for the horal fish. The present study
attempts to develop the DNA vaccines for protect NNV infection in marine fish. It is successful
to extract viral RNA and amplify a 1330 bp product using specific primers (NNV-F&R) against
NNV RNA2 by RT-PCR. After gene cloning, the purified plasmid that contained viral capsid
protein genome was used as DNA vaccine and transfected into GBC1 cell line by lipofectin or
Electroporation. The protection efficacy of DNA vaccine is then evaluated using rat anti-NNV
antibody by western blot hybridization method. It is verified that the transfected cell produce
42KDa protein is same as the molecular weight of NNV capsid protein. Therefore, it is hopeful
that developed DNA vaccine may be beneficial to grouper aquaculture industry in Taiwan.
Publisher
臺北市：國立臺灣大學漁業科學研究所
Type
journal article
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